Protein folding is a highly complex process, if you reverse a thermal process you almost certainly never get the same shape back. Thats one of the many reasons living things don't react well to large deviations from their nominal temperature. If I drop a glass on the floor then it will smash. Its very unlikly that anything I do to the shards of glass will yield the original glass. Even melting down will give a subtly different glass.
That's because all of those actions are initiated from a point in time at which the glass is broken. If the glass were intact in the future, and somebody initated an action backwards in time to break it, then it would be broken now. The effect, from our perspective, would be reassembly of the pieces.